Microscale assay for the quantification of total polysaccharides to estimate extracellular polymeric substances (EPS) in soil
We present an inexpensive and practical microscale spectrophotometric acid-phenol assay to quantify polysaccharides in aqueous solution. The instant reaction within disposable materials is designed and benchmarked for use in quantifying extracellular polymeric substances (EPS) in soils. Precipitation recovery was high across expected ranges of soil EPS content.
Authors
- Holly Vander Stel (ORCID: https://orcid.org/0000-0003-0077-3858)
- Jay T. Lennon (ORCID: https://orcid.org/0000-0003-3126-6111)
- Sarah E. Evans (ORCID: https://orcid.org/0000-0001-6728-4499)
- Glade D. Bogar (ORCID: https://orcid.org/0000-0002-1601-6336)
Institutions
- Carnegie Department of Plant Biology (US)
- Indiana University Bloomington (US)
- University of California, Davis (US)
- Michigan State University (US)
Publication Details
- Journal
- Journal of Microbiological Methods
- Published
- 2025-11-11
- DOI
- https://doi.org/10.1016/j.mimet.2025.107324
- Primary Topic
- Microplastics and Plastic Pollution
- Type
- article
- Field-Weighted Citation Impact
- 0.00