Comprehensive transcriptomic analysis revealing that 2-azahypoxanthine treatment induces the stress response in <i>Arabidopsis thaliana</i>

Some fungi form fairy rings by secreting fairy chemicals that regulate plant growth. Among them, 2-azahypoxanthine (AHX) has been proposed as a new phytohormone, but its mechanisms remain unclear. We treated Arabidopsis thaliana with 400 μm AHX and observed growth inhibition, contrasting with earlier reports in bentgrass. To elucidate this effect, we performed transcriptome sequencing and identified differentially expressed genes. Gene ontology enrichment revealed activation of hormone- and stress-related terms and suppression of photosynthesis-related terms. Liquid chromatography-mass spectrometry further showed an increase in 1-aminocyclopropanecarboxylic acid, an ethylene precursor, after AHX treatment. These results suggest that AHX alters the stress response in A. thaliana by regulating genes associated with multiple plant hormones, including abscisic acid, ethylene, jasmonic acid, salicylic acid, and cytokinins.

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Journal
Bioscience Biotechnology and Biochemistry
Published
2025-11-10
DOI
https://doi.org/10.1093/bbb/zbaf162
Primary Topic
Plant-Microbe Interactions and Immunity
Type
article
Field-Weighted Citation Impact
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article

Comprehensive transcriptomic analysis revealing that 2-azahypoxanthine treatment induces the stress response in Arabidopsis thaliana

Tomohiro Suzuki, Hitoshi Sakakibara, T. Nomura, Hitoshi Ouchi et al.
Bioscience Biotechnology and Biochemistry
Plant-Microbe Interactions and Immunity
article

Comprehensive transcriptomic analysis revealing that 2-azahypoxanthine treatment induces the stress response in Arabidopsis thaliana

Tomohiro Suzuki, Hitoshi Sakakibara, T. Nomura, Hitoshi Ouchi, Masanori Okamoto, Yuki Tanaka, Hideo Dohra, Mikiko Kojima, Makoto Inai, Jae‐Hoon Choi, Hirokazu Kawagishi, Yumiko Takebayashi, Keisuke Mitsukuni
article en

Abstract

Some fungi form fairy rings by secreting fairy chemicals that regulate plant growth. Among them, 2-azahypoxanthine (AHX) has been proposed as a new phytohormone, but its mechanisms remain unclear. We treated Arabidopsis thaliana with 400 μm AHX and observed growth inhibition, contrasting with earlier reports in bentgrass. To elucidate this effect, we performed transcriptome sequencing and identified differentially expressed genes. Gene ontology enrichment revealed activation of hormone- and stress-related terms and suppression of photosynthesis-related terms. Liquid chromatography-mass spectrometry further showed an increase in 1-aminocyclopropanecarboxylic acid, an ethylene precursor, after AHX treatment. These results suggest that AHX alters the stress response in A. thaliana by regulating genes associated with multiple plant hormones, including abscisic acid, ethylene, jasmonic acid, salicylic acid, and cytokinins.

Bioscience Biotechnology and BiochemistryVol. 90(1)
Shizuoka University (JP), University of Shizuoka (JP), Utsunomiya University (JP), RIKEN Center for Sustainable Resource Science (JP), Fukushima University (JP), Nagoya University (JP)
Openalex Percentile: Top 75%
Plant-Microbe Interactions and Immunity
DOISourceOpenAlex
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