Decoding HuH-7: a comprehensive genetic and molecular portrait of a widely used hepatocellular carcinoma model
Our results emphasize the importance of regular identity verification, such as short tandem repeat (STR) profiling, and demonstrate how subtle genetic variations can affect phenotypic traits relevant to modeling liver disease. By providing insights into the genetic and transcriptomic features of HuH-7 cells, this study establishes a robust basis for future research and therapeutic investigations using this widely accepted liver cell model. It also emphasizes the importance for maintaining high-quality standards and robust authentication practices to ensure that cell-based studies produce reliable and reproducible results.
Authors
- Eva Miriam Buhl (ORCID: https://orcid.org/0000-0003-0627-9228)
- Thomas Liehr (ORCID: https://orcid.org/0000-0003-1672-3054)
- Diandra T. Keller (ORCID: https://orcid.org/0009-0000-2266-3698)
- Ralf Weiskirchen (ORCID: https://orcid.org/0000-0003-3888-0931)
- Katja Hardt
- Anja Weise (ORCID: https://orcid.org/0000-0003-3988-8919)
- Constanze Pentzold (ORCID: https://orcid.org/0000-0003-3711-8945)
- Stefanie Kankel (ORCID: https://orcid.org/0000-0003-1042-5267)
- Sarah K. Schröder (ORCID: https://orcid.org/0000-0003-2265-3726)
- Daniela Luís
Institutions
- Universitätsklinikum Aachen (DE)
- Jena University Hospital (DE)
- Friedrich Schiller University Jena (DE)
- RWTH Aachen University (DE)
Publication Details
- Journal
- Frontiers in Cell and Developmental Biology
- Published
- 2025-11-11
- DOI
- https://doi.org/10.3389/fcell.2025.1648639
- Citations
- 2
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- RWTH Aachen University
- Medizinische Fakultät, RWTH Aachen University