The effect of using effective micro-organisms on the histopathological, immunohistochemical expression of kidneys in quails under thermal stress

Heat stress in chicken husbandry is substantial stressor factor with increasing environmental temperature its prominence will increasing. To investigate the renal tissue histopathological finding and immunohistochemical expression of interleukin-1beta (IL-1B) in the renal tissue of quails exposed to stress factor and supplemented with effective microorganism (EM). Forty one-day -old quails were utilized which were assign randomly into 4 groups of 10 quails every in each: M1 (control groups), M2 exposed to heat stress (36 C for sixty hour), M3 were giving both (EM and exposed to stress) and M4 remedy with Effective Microorganism (EM) 1000ppm in drinking water, 5 birds from each group were sacrificed at the end of experiment (20 and 40days respectively), the kidney was weighed, then 4-5nµ sections were prepared, stained with haematoxylin and eosin. Immunohistochemistry procedures were applied according to manufacturer’s protocol. Microscopic finding was identified by infiltration of inflammatory cell, bleeding, glomerular atrophy, and glomerular necrosis, sever destruction of tubular epithelium, coagulate necrosis, pyknosis and hyper cellularity, kidney relative weights was higher in Effective Microorganism group than controls and others groups, IL-1B cytokine protein expression was higher at 40 days of the experiment in contrast to first 20 days of it. From this result were concluded that although quails are resistant to environmental conditions, thermal stress had a negative impact on the study parameters.