Utilization of cell-free DNA metagenomic analysis for early detection and microbial identification in prosthetic joint infections: a prospective cohort study in Korea
cfDNA concentration was significantly higher in the PJI group, with synovial cultures showing substantial agreement. Additionally, cfDNA sequencing detected pathogens in patients who had received prior antibiotic therapy and identified multiple pathogens in polymicrobial infections. These findings highlight cfDNA analysis as a valuable diagnostic tool for PJI, with the potential to enhance current diagnostic approaches.
Authors
- Dongju Won (ORCID: https://orcid.org/0000-0002-0084-0216)
- Kwan Kyu Park (ORCID: https://orcid.org/0000-0001-5117-5853)
- Seung‐Tae Lee (ORCID: https://orcid.org/0000-0003-1047-1415)
- Eun Hwa Lee (ORCID: https://orcid.org/0000-0002-1182-1977)
- Saeam Shin
- Jung Ah Lee (ORCID: https://orcid.org/0000-0002-5837-3008)
- Su Jin Jeong
Institutions
- Severance Hospital (KR)
- Gangnam Severance Hospital (KR)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-11-10
- DOI
- https://doi.org/10.3389/fcimb.2025.1663857
- Primary Topic
- Orthopedic Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- Yonsei University
- National Research Foundation of Korea
- Yonsei University College of Medicine