Optimization of donor structure enhances the generation of cloned goats with high expression of human butyrylcholinesterase by CRISPR/Cas9
Optimizing donor structure improves precise genome editing efficiency and enables robust generation of transgenic goats. This strategy advances CRISPR/Cas9-based bioreactor development for scalable production of therapeutic proteins.
Authors
- Yunzhi Fa
- Rui Zhang (ORCID: https://orcid.org/0000-0002-9238-3621)
- T. Sun (ORCID: https://orcid.org/0000-0003-2994-4526)
- Yan Li (ORCID: https://orcid.org/0000-0002-2091-7313)
- Yunpeng Wu
- Yefeng Qiu (ORCID: https://orcid.org/0000-0002-5392-3293)
- Jianhua Zheng
- Jing‐qing Chen (ORCID: https://orcid.org/0009-0000-0222-8605)
- Tao Luan (ORCID: https://orcid.org/0009-0005-4584-4121)
Institutions
- Academy of Military Medical Sciences (CN)
- Mudanjiang Normal University (CN)
- Beijing Advanced Sciences and Innovation Center (CN)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-11-10
- DOI
- https://doi.org/10.3389/fbioe.2025.1633553
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00