Two New Chromone Derivatives from a Marine Algicolous Fungus Aspergillus versicolor GXIMD 02518 and Their Osteoclastogenesis Inhibitory Activity

Two new chromone derivatives, cnidimols I and J (<b>1</b> and <b>2</b>), together with ten known aromatic derivatives (<b>3</b>-<b>12</b>), were isolated from the Beibu Gulf algicolous fungus <i>Aspergillus versicolor</i> GXIMD 02518. Their structures were determined by comprehensive physicochemical and spectroscopic data interpretation. The absolute configurations of <b>1</b> and <b>2</b> were accomplished by ECD calculations and X-ray diffraction analysis. Compound <b>1</b> was obtained as a pair of enantiomers, which were separated by chiral-phase HPLC analysis. Notably, 3,7-dihydroxy-1,9-dimethyldibenzofuran (<b>6</b>) displayed significant inhibition in LPS-induced NF-κB luciferase activity in RAW 264.7 macrophages, which further inhibited RANKL-induced osteoclast differentiation without cytotoxicity in bone marrow macrophage cells.