Overexpression of AdeIJK RND efflux pump in <i>Acinetobacter baumannii</i> using the mini-Tn <i> <b>7</b> </i> T-based single copy induction system can lead to <i> lacI ^q </i> mutation

Genetic tools are essential for gaining deeper insights into bacterial systems. Though these tools have been of great value in the study of genotype-phenotype relationships, it is equally important to recognize their limitations. We believe this observation will help raise awareness of a potential constraint associated with the widely used mini-Tn7 gene expression system and enable researchers to avoid misinterpretation of experimental results. Being conscious of this limitation, subtle changes that may have otherwise gone unnoticed can be more effectively identified.