Phytofabrication of ZIF-8 Using Mangrove Metabolites for Dual Action Against Drug-Resistant Microbes and Breast Cancer Cells

Green nanotechnology offers a sustainable and eco-friendly approach for nanoframework synthesis. The present study intended to synthesize a novel eco-friendly encapsulated Zeolitic Imidazolate Framework-8 (ZIF-8) in a one-pot method using metabolites from the mangrove plant <i>Conocarpus erectus</i> (CE). Gas Chromatography-Mass Spectrometry (GC-MS) analysis of the extract revealed the presence of important bioactive metabolites. The synthesized material was evaluated by UV-Vis spectroscopy, X-ray diffraction (XRD), particle size analysis (PSA), zeta potential measurement, high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared (FT-IR) spectroscopy studies. The environment-friendly mangrove metabolites aided by Zeolitic Imidazolate Framework-8 was found to be crystalline, rhombic dodecahedron structured, and size dispersed without agglomeration. The nanomaterial possessed a broad antimicrobial effect on drug-resistant microorganisms, including <i>Candida krusei</i>, <i>Escherichia coli</i>, <i>Streptococcus</i> Sp., <i>Staphylococcus aureus</i>, <i>Enterococcus</i> Sp., <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, <i>C. propicalis</i>, and <i>C. albicans</i>. Further, its cytotoxicity against MDA-MB-231 cells was found to be efficient. The morphological alterations exhibited by the antiproliferative impact on the breast cancer cell line were detected using DAPI and AO/EB staining. Therefore, ZIF-8 encapsulated mangrove metabolites could serve as an effective biomaterial with biomedical properties in the future.