Cost-Effective Method for Using Cross-Species Spike-In RNA for Normalization and Quantification in Polysome Profiling Experiments
Using total RNA from a non-related species as a spike-in control offers a practical, economical alternative to conventional methods. This approach enhances the reliability of RNA quantification without compromising experimental integrity, making it especially valuable for resource-limited settings, particularly in polysome and RT-qPCR workflows.
Authors
- Angelo Slade
- Martin Holčı́k (ORCID: https://orcid.org/0000-0002-1922-5299)
- Krishna Bhattarai (ORCID: https://orcid.org/0000-0002-6787-8008)
Institutions
- Carleton University (CA)
Publication Details
- Journal
- Genes
- Published
- 2025-11-10
- DOI
- https://doi.org/10.3390/genes16111354
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Sciences and Engineering Research Council of Canada