An Effective Process to Use Drones for Above-Ground Biomass Estimation in Agroforestry Landscapes

Above-ground biomass in agroforestry refers to the total mass of living vegetation, primarily trees and shrubs, integrated into agricultural landscapes. It plays a key role in climate change mitigation by capturing and storing carbon. Accurate estimation of above-ground biomass in agroforestry systems requires effective drone deployment and sensor management. This study presents a detailed methodology for biomass estimation using Unmanned Aircraft Systems, based on an experimental campaign conducted in the Campania region of Italy. Multispectral drone platforms were used to generate calibrated reflectance maps and derive vegetation indices for biomass estimation in agroforestry landscapes. Integrating field-measured tree attributes with remote sensing indices improved the accuracy and efficiency of biomass prediction. Following the assessment of mission parameters, flights were conducted using a commercial drone to demonstrate consistency of results across multiple altitudes. Terrain-follow mode and high image overlap were employed to evaluate ground sampling distance sensitivity, radiometric performance, and overall data quality. The outcome is a defined process that enables agronomists to effectively estimate above-ground biomass in agroforestry landscapes using drone platforms, following the procedure outlined in this paper. Predictive performance was evaluated using standard model metrics, including R2, RMSE, and MAE, which are essential for replicability and comparison in future studies.