Visual Detection of H <sub>2</sub> O <sub>2</sub> in Serum Based on Covalent G-Quadruplex Hemin
This work presents a G-quadruplex heme chloride DNA peroxidase biosensing system. To address the instability and strong background signal issues in visual H<sub>2</sub>O<sub>2</sub> detection, a G-quadruplex was prepared by using four trimeric DNA strands, and hemin was attached via covalent binding. The covalent binding significantly improved the color rendering effect and reduced the background signal. Four types of single-stranded DNA were compared for catalytic activity, with (GGGA)3 showing the best performance. The system was successfully applied to detect H<sub>2</sub>O<sub>2</sub> in human serum, reaching a minimum detection concentration at the nanomolar level. This study provides valuable data for selecting single-stranded DNA in G-quadruplex preparation and holds potential for visual H<sub>2</sub>O<sub>2</sub> detection in biomedicine and various industrial fields.
Authors
- Yun Bai (ORCID: https://orcid.org/0000-0002-2688-9229)
- Jun Ai (ORCID: https://orcid.org/0000-0002-3093-4539)
- Lu Ga
- Ailing Su
- Feiyang Bai (ORCID: https://orcid.org/0009-0007-2382-778X)
- Denggerile Ao
Institutions
- Inner Mongolia Normal University (CN)
- Jinchuan (China) (CN)
- Inner Mongolia Medical University (CN)
Publication Details
- Journal
- ACS Applied Bio Materials
- Published
- 2025-11-09
- DOI
- https://doi.org/10.1021/acsabm.5c01348
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00