Phenolic Compounds from <i>Diarthron iranica</i>: Enzymatic and in Silico Insights Into α-Amylase Inhibitory Activity
The phytochemical analysis carried out on the aerial parts of <i>D. iranica</i> yielded the identification and characterization of four phenolic compounds, including a methoxy apigenin glycoside (1), one bicoumarin (2), and two lignans (3-4). Molecular docking studies indicated that compound 1 exhibited superior inhibitory potential compared to compound 3, with stable interactions in the enzyme's binding site. In α-amylase inhibitory assays, these compounds displayed varying levels of activity, with compound 2 showing the highest potency (IC<sub>50</sub> = 0.71 mg/mL), followed by compounds 1 (IC<sub>50</sub> = 1.32 mg/mL) and 3 (IC<sub>50</sub> = 1.81 mg/mL). However, all were less effective than the reference compound luteolin (IC<sub>50</sub> = 0.63 mg/mL), which demonstrated superior efficacy.
Authors
- Morteza Sadeghi (ORCID: https://orcid.org/0000-0002-5027-4777)
- Mustafa Ghanadian (ORCID: https://orcid.org/0000-0001-6446-4734)
- Zeinab Yazdiniapour (ORCID: https://orcid.org/0000-0003-0102-4306)
- Mehran Miroliaei
- Reza Gashavi
- Tohid Khodabande
- Hossein Biganeh
Institutions
- Isfahan University of Medical Sciences (IR)
- Islamic Azad University Sanandaj Branch (IR)
- University of Isfahan (IR)
Publication Details
- Journal
- Iranian journal of pharmaceutical research
- Published
- 2025-11-04
- DOI
- https://doi.org/10.5812/ijpr-164807
- Primary Topic
- Flavonoids in Medical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Isfahan University of Medical Sciences