Unravelling the triad of penicillin-binding proteins, β-lactamase activity, and mRNA dynamics in <i>Pseudomonas aeruginosa</i> AmpC induction
These findings highlight that ampC transcription does not predict functional enzyme levels, especially for PBP3-binding drugs. Full induction appears to require PBP4 targeting and CreBC activation. These findings underscore the need for integrated assessment of PBP IC50, transcription, and enzymatic activity to guide rational β-lactam and β-lactamase inhibitor therapy, and lay the groundwork for future studies on alternative ampC regulatory pathways.
Authors
- Antonio Oliver (ORCID: https://orcid.org/0000-0001-8571-2733)
- Marisa Montes (ORCID: https://orcid.org/0000-0003-1748-813X)
- Bartolomé Moyá (ORCID: https://orcid.org/0000-0001-6404-246X)
- María Montaner (ORCID: https://orcid.org/0000-0002-7335-9781)
- Silvia López-Argüello (ORCID: https://orcid.org/0000-0001-6782-2947)
- Francina Alajarin
Institutions
- Centro de Investigación Biomédica en Red (ES)
- Health Research Institute of the Balearic Islands (ES)
- Hospital Universitario Son Espases (ES)
Publication Details
- Journal
- Journal of Antimicrobial Chemotherapy
- Published
- 2025-11-05
- DOI
- https://doi.org/10.1093/jac/dkaf408
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.00