The mechanism of black ginseng extract in alleviating inflammatory aging

Black ginseng is a unique processed product of ginseng, a source of health food, medicine and modern cosmetics. The purpose of this study was to develop a new application for preventing inflammatory aging. To investigate the effects and mechanisms of action of black ginseng extract (BGE) on inflammatory aging, models of inflammatory aging were established in human foreskin fibroblasts cells and reconstructed 3D skin model. The results showed that MMP-1 expression in human foreskin fibroblasts was reduced at a concentration of 4 μg/mL BGE. At a concentration of 0.1%, BGE not only inhibited the secretion of PGE 2 in reconstructed 3D skin model damaged by SDS but also promoted the expression of TIMP-1. Furthermore, the composition of three rare ginsenosides in the BGE and their binding affinity to target proteins associated with inflammatory aging were analyzed using high-performance liquid chromatography (HPLC) and molecular docking technique. In summary, BGE has potential application value in alleviating skin inflammatory aging.