Breaking through PCR multiplexing barriers: A shared-label-tagged primer design enables high-efficiency single-reaction booster PCR (SLP-bPCR)
The SLP-bPCR technology establishes a transformative framework for multiplex nucleic acid detection, delivering unprecedented analytical performance through its unique combination of versatility, efficiency, and scalability. By addressing critical gaps in throughput, accuracy, and practicality, this strategy is poised to advance both high-throughput research and clinical diagnostics, particularly for applications demanding multi-analyte profiling at extreme sensitivity.
Authors
- Ling Zhu (ORCID: https://orcid.org/0000-0002-7960-555X)
- Guoqing Deng
- Yong Liu (ORCID: https://orcid.org/0000-0002-2966-7867)
- Ke Yang (ORCID: https://orcid.org/0000-0003-4964-8214)
- Jun Zhao (ORCID: https://orcid.org/0000-0002-4800-7776)
Institutions
- Hefei Institutes of Physical Science (CN)
- Anhui Institute of Optics and Fine Mechanics (CN)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2025-11-08
- DOI
- https://doi.org/10.1016/j.aca.2025.344869
- Primary Topic
- Innovative Microfluidic and Catalytic Techniques Innovation
- Type
- article
- Field-Weighted Citation Impact
- 0.00