Performance of metagenomic next-generation sequencing in bronchoalveolar lavage fluid for pathogen detection in patients with acute exacerbations of bronchiectasis
This study aimed to evaluate the diagnostic value of metagenomic next-generation sequencing (mNGS) of bronchoalveolar lavage fluid (BALF) in detecting pathogens among bronchiectasis patients with acute exacerbations. A retrospective analysis was conducted on 89 bronchiectasis patients who were treated for acute exacerbations at the First Affiliated Hospital of Ningbo University from April 1, 2021, to September 30, 2023. Among the 89 patients, 88 were diagnosed with pulmonary infection, of which 15.9% (14/88) were cases of mixed infections. The sensitivity of BALF-mNGS for detecting pathogens in bronchiectasis patients during acute exacerbations was significantly higher than that of BALF culture (93.2% vs 28.4%; P < .001). All cases of mixed infection were fully identified by BALF-mNGS. The most common pathogens in patients with bronchiectasis were Pseudomonas aeruginosa, nontuberculous mycobacteria, Haemophilus influenzae, and Aspergillus. In conclusion, compared with the traditional microbial culture method, BALF-mNGS significantly improves the accuracy of diagnosis for detecting pathogens associated with bronchiectasis infections.
Authors
- Hui Chen (ORCID: https://orcid.org/0000-0002-7689-400X)
- Qidong Zhuang (ORCID: https://orcid.org/0000-0002-5585-0980)
- Xiaoqing Pan (ORCID: https://orcid.org/0000-0002-0965-8568)
- Lin Zheng (ORCID: https://orcid.org/0000-0001-7821-6985)
- Yiming Yu (ORCID: https://orcid.org/0000-0002-9919-7282)
- Xinying Sun (ORCID: https://orcid.org/0000-0001-6638-1473)
- Zaichun Deng (ORCID: https://orcid.org/0000-0002-4562-2124)
- Lihong Wan (ORCID: https://orcid.org/0000-0003-0086-8857)
- Xuechan Yu (ORCID: https://orcid.org/0000-0003-2124-199X)
- Ruyi Xu (ORCID: https://orcid.org/0000-0002-9538-1445)
- Sha Li (ORCID: https://orcid.org/0000-0002-1006-1785)
- Zhongbo Chen (ORCID: https://orcid.org/0000-0002-8477-9546)
- Xiaoxuan Zheng (ORCID: https://orcid.org/0009-0006-5986-0365)
Institutions
- Ningbo University (CN)
- Shanghai Jiao Tong University (CN)
- Shanghai Chest Hospital (CN)
- Ningbo No. 2 Hospital (CN)
- Zhuhai People's Hospital (CN)
- Chinese People 's Liberation Army No.455 Hospital (CN)
- Chinese People 's Liberation Army No. 85 Hospital (CN)
- Ningbo University Affiliated Hospital (CN)
- Second Affiliated Hospital of Zhejiang University (CN)
- Chinese People's Liberation Army (CN)
- Ningbo No.6 Hospital (CN)
- Ningbo First Hospital (CN)
Publication Details
- Journal
- Medicine
- Published
- 2025-11-07
- DOI
- https://doi.org/10.1097/md.0000000000045606
- Citations
- 1
- Primary Topic
- Cystic Fibrosis Research Advances
- Type
- article
- Field-Weighted Citation Impact
- 1.52