Graphene Oxide-Based Acetamiprid Detection Platform and the Construction of Logic Gate

Pesticides are widely used in food cultivation and have become one of the most important means of ensuring the development of agriculture, forestry and animal husbandry. At the same time, pesticide residue detection technology needs to be updated to provide a scientific and effective basis to cope with the problems arising from the use of pesticides. As a new type of nicotine insecticide, acetamiprid is widely used in agricultural production. This paper reports a fluorescent biosensor based on graphene oxide and G-quadruplex that can be used for the determination of the target acetamiprid. It contains a specific DNA probe that can form the G-quadruplex structure and the nucleic acid sequence of the acetamiprid aptamer as the main element. The probe is stabilized and adsorbed by the $\pi $ - $\pi $ interaction of graphene, achieving the variation of the assay results. The optimal sequences designed for rapid screening of nucleic acids were modeled and simulated using bioinformatics tools such as NUPACK and AutoDock prior to the experiment. The detection limit of acetamiprid was 165.5 pM, which was much lower than the national food safety standard residue of 0.05 mg/kg. The sensor has the advantages of obvious economic value, fewer steps and shorter detection time. It has great application prospects in the field of food safety.