A Multicomponent Salmon PDRN/Sodium Alginate/Gelatin Hydrogel Promotes Infected Wound Repair in Rat

Infected wound healing is a clinical problem owing to excessive inflammatory responses. In this study, a multicomponent hydrogel GOP was prepared by employing salmon polydeoxyribonucleotide (PDRN), oxidized sodium alginate (OSA), and gelatin (Gel) as starting materials. The hydrogel with three-dimensional structure was synthesized through the Schiff-base cross-linking of PDRN, Gel, and OSA. Hydrogen bonding and electrostatic interaction also participated in the formation of the three-dimensional network. Hydrogel GOP demonstrated a good mechanical property and tissue adhesion ability. In vitro studies revealed the antibacterial activity of hydrogel GOP toward <i>S. aureus</i> and <i>E. coli</i>, along with remarkable antioxidant activity. Biocompatibility tests showed high cell viability (>80%) and good ability to promote cell migration. Hemolysis assay revealed minimal hemotoxicity (<2.1%). Using a murine full-thickness dermal injury model, GOP treatment achieved small residual wound area (2.3%) on day 14. Histological analyses demonstrated reduced inflammation cells and enhanced collagen deposition. Immunofluorescence analyses showed suppressed inflammation level (increased IL-10) and improved angiogenesis (upregulated CD31 and α-SMA). These results revealed that hydrogel GOP represented a promising multifunctional dressing for infected wound management.