Electron‐Mediator‐Free Microfluidic Photocatalytic Coenzyme Regeneration with 100% Conversion Efficiency within 126 S
Microfluidic systems enhance photocatalytic efficiency through superior mass/energy transfer and precise parameter control. Here, an electron-mediator-free microfluidic platform is designed for photocatalytic coenzyme NAD(P)H regeneration by integrating ultrathin BiOBr nanosheets. This architecture enables direct electron-proton coupling, achieving 100% NAD<sup>+</sup> conversion within 126 s with exceptional selectivity (72.30%) for bioactive 1,4-NADH. Continuous operation over 32 h shows no activity decay, demonstrating unparalleled stability. This study establishes a benchmark for designing integrated photocatalytic systems and highlights their broad potential for applications in biocatalysis, synthetic biology, and renewable energy.
Authors
- Chi Chung Tsoi (ORCID: https://orcid.org/0000-0003-1122-4889)
- Jinlin Long (ORCID: https://orcid.org/0000-0002-3675-0941)
- Zirui Pang
- Xuming Zhang (ORCID: https://orcid.org/0000-0002-9326-5547)
- Fengjia Xie
- Huaping Jia (ORCID: https://orcid.org/0000-0003-0693-2573)
- Zizhong Zhang (ORCID: https://orcid.org/0000-0002-9541-8981)
- Zixuan Jia (ORCID: https://orcid.org/0000-0002-6713-9942)
- Jinni Shen (ORCID: https://orcid.org/0000-0002-6330-9107)
- Liang Wan
- Heng Jiang (ORCID: https://orcid.org/0000-0001-9958-9350)
- Li Xu (ORCID: https://orcid.org/0000-0002-5867-0651)
- Yao Chai (ORCID: https://orcid.org/0000-0002-0967-7945)
- Yanmei Chen (ORCID: https://orcid.org/0000-0003-3226-031X)
Institutions
- Hong Kong Polytechnic University (HK)
- Fuzhou University (CN)
Publication Details
- Journal
- Advanced Science
- Published
- 2025-11-07
- DOI
- https://doi.org/10.1002/advs.202513720
- Primary Topic
- Metalloenzymes and iron-sulfur proteins
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Innovation and Technology Commission
- Hong Kong Polytechnic University
- Medical Research Council
- Division of Materials Research