Single-nucleus profiling highlights the all-brain echinoderm nervous system
Metazoans comprise diverse tissues and cell types, each essential for the organismal survival. Most of these types are established early in embryogenesis and persist into adulthood. In indirectly developing sea urchins, however, the continuity between embryonic and adult stages is interrupted by a planktonic larval stage that undergoes complete metamorphosis. While gene regulatory networks controlling embryonic and larval lineages are well studied, the molecular and morphological identities of postmetamorphic cell types remain poorly understood. Here, we reconstructed the cell atlas of postmetamorphic <i>Paracentrotus lividus</i> juveniles using single-nucleus transcriptomics, revealing conservation of regulatory mechanisms. We identified signatures of eight distinct cell type groups and analyzed 29 neuronal families, including 15 unique photoreceptor types. By combining transcriptomics, spatial analysis, and ultrastructure, we identified vertebrate neuronal and opsin homologs expressed across the juvenile. These findings show that the echinoderm body plan is predominantly head-like and exhibits an "all-brain" organization.
Authors
- Filomena Caccavale
- Maria Cocurullo (ORCID: https://orcid.org/0000-0002-7729-3281)
- Carsten Lüter (ORCID: https://orcid.org/0000-0003-1544-9460)
- Jil Carl (ORCID: https://orcid.org/0000-0002-9413-2375)
- Alba Almazán (ORCID: https://orcid.org/0000-0001-9933-4278)
- Giovanna Benvenuto (ORCID: https://orcid.org/0000-0001-7155-2935)
- Maria Schauer
- Tiphaine Sancerni (ORCID: https://orcid.org/0009-0006-7409-2181)
- Periklis Paganos (ORCID: https://orcid.org/0000-0001-9525-4625)
- Jenifer C. Croce (ORCID: https://orcid.org/0000-0001-7092-0257)
- Anne-C. Zakrzewski (ORCID: https://orcid.org/0000-0001-8868-1696)
- Oğuz Akar
- Maria Ina Arnone (ORCID: https://orcid.org/0000-0002-9012-7624)
- Danila Voronov (ORCID: https://orcid.org/0000-0002-2972-6484)
- Berit Zemann (ORCID: https://orcid.org/0009-0008-2293-3588)
- Jack Ullrich-Lüter (ORCID: https://orcid.org/0000-0002-2535-2577)
- Maria Lorenza Rusciano (ORCID: https://orcid.org/0009-0000-6428-2674)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- Museum für Naturkunde (DE)
- Stazione Zoologica Anton Dohrn (IT)
- Sorbonne Université (FR)
- Institut de Génomique Fonctionnelle de Lyon (FR)
- Laboratoire de Biologie du Développement de Villefranche-sur-Mer (FR)
- Institut de la Mer de Villefranche (FR)
Publication Details
- Journal
- Science Advances
- Published
- 2025-11-05
- DOI
- https://doi.org/10.1126/sciadv.adx7753
- Citations
- 2
- Primary Topic
- Developmental Biology and Gene Regulation
- Type
- article
- Field-Weighted Citation Impact
- 1.61