Salivary Biomarker Profile in Periodontal Diseases: A Cross-Sectional Study on Leptin, Adiponectin, and Calprotectin

<b>Background/Objectives</b>: This study aimed to evaluate salivary leptin, adiponectin, and calprotectin levels and to investigate the associations among these biomarkers in periodontally healthy individuals, as well as in patients with gingivitis and periodontitis. <b>Methods</b>: A total of 165 participants were included: 55 periodontally healthy individuals, 55 with gingivitis, and 55 with periodontitis. Unstimulated saliva was collected via passive drool, and salivary leptin, adiponectin, and calprotectin levels were biochemically quantified using enzyme-linked immunosorbent assay. <b>Results</b>: Salivary leptin levels were significantly lower in the periodontally healthy group than those in the gingivitis and periodontitis groups, whereas adiponectin levels were reduced in the periodontitis group than in the periodontally healthy and gingivitis groups (<i>p</i> < 0.05). Salivary calprotectin levels differed significantly among groups, highest in the periodontitis group, followed by the gingivitis and periodontally healthy groups (<i>p</i> < 0.05). Salivary leptin and calprotectin levels demonstrated significant positive correlations with all clinical periodontal parameters, while adiponectin levels were negatively correlated (<i>p</i> < 0.05). Receiver operating characteristic and logistic regression analyses identified salivary leptin, calprotectin, and adiponectin levels as significant biomarkers for distinguishing periodontal health, gingivitis, and periodontitis (<i>p</i> < 0.05). <b>Conclusions</b>: These findings suggest salivary leptin, calprotectin, and adiponectin may serve as biomarkers and potential risk predictors of periodontal disease.