Environmental stiffness regulates neuronal maturation via Piezo1-mediated transthyretin activity
During development, neurons initiate a maturation process during which they start expressing voltage-gated ion channels, form synapses, and start communicating via action potentials. Little is known about external factors regulating this process. Here, we identify environmental mechanics as an important regulator of neuronal maturation, and a molecular pathway linking tissue stiffness to this process. Using patch clamp electrophysiology, calcium imaging and immunofluorescence, we find that, in stiffer environments, neurons show a delay in voltage-gated ion channel activity, action potentials, and synapse formation. RNA sequencing and CRISPR/Cas9 knockdown reveal that the mechanosensitive ion channel Piezo1 supresses transthyretin expression on stiffer substrates, slowing down electrical maturation. In Xenopus laevis embryos, brain stiffness negatively correlates with synapse density, and artificial tissue stiffening delays synaptic activity in vivo. Our data indicate that environmental stiffness represents a fundamental regulator of neuronal maturation, critical for brain circuit development and potentially for neurodevelopmental disorders.
Authors
- Kristian Franze (ORCID: https://orcid.org/0000-0002-8425-7297)
- Katrin A. Mooslehner
- Eva K. Pillai (ORCID: https://orcid.org/0000-0002-4662-3356)
- Sudipta Mukherjee (ORCID: https://orcid.org/0000-0003-3209-7399)
- Robert Humphrey
- Ragnhildur Thóra Káradóttir (ORCID: https://orcid.org/0000-0001-9675-2722)
- Eva Kreysing (ORCID: https://orcid.org/0000-0002-7984-2117)
- Leila Mureşan (ORCID: https://orcid.org/0000-0002-7602-0249)
- Andrea Dimitracopoulos (ORCID: https://orcid.org/0000-0001-6776-4214)
- Xiaohui Zhao (ORCID: https://orcid.org/0000-0001-9922-2815)
- Alexander K. Winkel (ORCID: https://orcid.org/0000-0001-9400-7964)
- Hélène Gautier (ORCID: https://orcid.org/0000-0002-3302-9470)
- Sebastián Vásquez-Sepúlveda
- Niklas Gampl (ORCID: https://orcid.org/0009-0002-1392-0108)
- Daniel Haarhoff
- Tina Borić (ORCID: https://orcid.org/0009-0003-6615-1127)
Institutions
- Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
- Wellcome/MRC Cambridge Stem Cell Institute (GB)
- University of Cambridge (GB)
- University of Warwick (GB)
- Cambridge School (PT)
- Lung Institute (US)
- Max Planck Institute for the Science of Light (DE)
- Stem Cell Institute (PA)
- Anglia Ruskin University (GB)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-11-07
- DOI
- https://doi.org/10.1038/s41467-025-64810-3
- Citations
- 2
- Primary Topic
- Erythrocyte Function and Pathophysiology
- Type
- article
- Field-Weighted Citation Impact
- 2.33
Funders
- Alexander von Humboldt-Stiftung
- Wellcome Trust
- Deutsche Forschungsgemeinschaft
- Cambridge Trust
- Medical Research Council
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council