Comparison and evaluation of metagenomic next-generation sequencing (mNGS) and real-time PCR for the detection of Mycobacterium tuberculosis
Both RT-PCR and mNGS demonstrate high overall agreement for MTB detection, with concordance strongly influenced by microbial burden. These findings support the complementary use of these methods in the diagnosis of TB.
Authors
- Xuegao Yu
- Jiankai Deng
- Peng Guo (ORCID: https://orcid.org/0000-0003-2656-9345)
- Yaoming Chen
- Yan‐Hong Wang (ORCID: https://orcid.org/0000-0003-3341-8991)
- Hongji Zhu (ORCID: https://orcid.org/0000-0001-5232-6844)
Institutions
- Sun Yat-sen University (CN)
- The First Affiliated Hospital, Sun Yat-sen University (CN)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-11-07
- DOI
- https://doi.org/10.3389/fcimb.2025.1694179
- Primary Topic
- Tuberculosis Research and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00