Photoelectrochemical assay for <i>E. coli</i> based on CBT–Cys-guided rolling circle amplification
A bacterial detection strategy combining CBT-Cys click ligation with enzyme-assisted amplification was fabricated. Padlock probe circularization and phi29-mediated amplification enabled multivalent aptamer generation for <i>E. coli</i> capture. Subsequently, β-galactosidase was released for producing <i>p</i>-aminophenol, and <i>E. coli</i> was detected sensitively by PEC.
Authors
- Wanfeng Zhang
- Anrui Liu
- Wen Li (ORCID: https://orcid.org/0000-0002-3851-7406)
- Jie Zheng (ORCID: https://orcid.org/0000-0002-2999-0103)
- Xu Hun (ORCID: https://orcid.org/0000-0001-9536-6790)
Institutions
- Ankang University (CN)
- Qingdao University of Science and Technology (CN)
- Xiamen University (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc05097a
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00