Molecular Detection of Neiseria Gonorrheae and Chlamydia Trichomatis Among Infertile Male Client Attending some Health Facilities in the Northwest, Nigeria

Background: The purpose of this study was to molecularly detect and evaluate the prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae infections among infertile men that attended some health facilities in some states of the Northwest, Nigeria using a multiplex molecular method, and the possible impact of C. trachomatis and N. gonorrhoeae infections on semen parameters was also noted in the present study. Methods: A total of 383 infertile male semen samples were collected for this study. C. trachomatis and N. gonorrhoeae infections were molecularly detected in the semen samples using the multiplex method. Semen parameters, risk factors, and age were analyzed and collated. Results: A total of 28 and 21 semen samples were found positive for C. trachomatis and N. gonorrhoeae, respectively, with a prevalence of C. trachomatis and N. gonorrhoeae relatively lower—7.3% and 5.5%, respectively. Those with primary infertility had the highest infections, with those with secondary infertility showing the least infection. Conclusions: Our study demonstrated a moderately medium prevalence of C. trachomatis and N. gonorrhoeae infection based on the multiplex PCR detection method. C. trachomatis and N. gonorrhoeae infection could possibly impair the male fertility factor through increased sperm DNA damage.