CAPRIN1 specifically mediates m6A modification of RIG-I RNA to inhibit Mycobacterium Tuberculosis infection
Tuberculosis (TB), the leading cause of death from a single infectious agent, remains incompletely understood in its pathogenic mechanisms. Through population-based bioinformatic analyses, we identified m<sup>6</sup>A modifications may play a critical role in the infection dynamics of tuberculosis, with CAPRIN1 emerging as a TB-specific m<sup>6</sup>A regulatory factor. Molecular and cellular experiments demonstrated that CAPRIN1 regulates the m<sup>6</sup>A modification of RIG-I RNA through direct interaction with METTL3, further influencing downstream interferon-associated gene networks and modulating Mycobacterium tuberculosis (M. tuberculosis) infection. Moreover, we discovered that these molecular biological processes predominantly occur within cellular stress granules (SGs). In summary, this study elucidates a CAPRIN1-specific m<sup>6</sup>A modification mechanism targeting RIG-I, proposing a potential target for the prevention and treatment of TB. and contributing to the theory of m<sup>6</sup>A's specific regulatory roles.
Authors
- Ruili Zheng (ORCID: https://orcid.org/0000-0002-3246-0269)
- Lijuan Zhou (ORCID: https://orcid.org/0000-0002-5826-3933)
- Hailong Wang (ORCID: https://orcid.org/0000-0001-9874-5892)
- Wei‐Te Lee (ORCID: https://orcid.org/0000-0002-9366-6180)
- Xiaoping Lv (ORCID: https://orcid.org/0000-0002-5296-2418)
- Peijiang Pan
- Yafei Chen (ORCID: https://orcid.org/0000-0002-2409-2048)
- Junjun Jiang (ORCID: https://orcid.org/0000-0003-4905-9348)
- Hubin Chen (ORCID: https://orcid.org/0009-0003-0216-7138)
- Hao Liang (ORCID: https://orcid.org/0000-0001-7534-5124)
- Jiemei Chu (ORCID: https://orcid.org/0000-0003-2942-0490)
- Jun Huang (ORCID: https://orcid.org/0000-0002-8402-9462)
- Sanqi An (ORCID: https://orcid.org/0000-0002-3177-213X)
- Yi Ling (ORCID: https://orcid.org/0000-0001-7392-8641)
- Shiou Yih Lee (ORCID: https://orcid.org/0000-0001-9493-1436)
- Li Ye (ORCID: https://orcid.org/0000-0001-7688-4867)
- Yanyan Liao (ORCID: https://orcid.org/0000-0003-1789-8091)
- Xinyue Xu
- Zheng Li (ORCID: https://orcid.org/0000-0002-1126-0428)
- Dan Jiang
Institutions
- INTI International University (MY)
- Guangxi University (CN)
- Guangxi Medical University (CN)
- Shinawatra University (TH)
- First Affiliated Hospital of GuangXi Medical University (CN)
- TCM-Intigrated Cancer Center of Southern Medical University (CN)
Publication Details
- Journal
- Communications Biology
- Published
- 2025-11-06
- DOI
- https://doi.org/10.1038/s42003-025-08897-w
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Guangxi Province