Rapid donor-specific single nucleotide variation detection by nanopore sequencing of ex vivo lung perfusate
Despite the lower sequencing accuracy and depth obtained from Nanopore sequencing, a high positive predictive value can be achieved in a set of donor-specific SNVs when appropriately filtered by read depth and overlap with SNP databases. This demonstrates the potential for the use of Nanopore sequencing to generate personalized donor cfDNA maps for use in post-operative donor-derived plasma cfDNA identification.
Authors
- Jared T. Simpson (ORCID: https://orcid.org/0000-0002-2116-2602)
- Philip C. Zuzarte (ORCID: https://orcid.org/0000-0002-0868-7455)
- Haruchika Yamamoto (ORCID: https://orcid.org/0000-0001-8975-3428)
- Jonathan Yeung (ORCID: https://orcid.org/0000-0001-5759-3028)
- Gavin W. Wilson (ORCID: https://orcid.org/0000-0002-3557-6320)
- Shaf Keshavjee (ORCID: https://orcid.org/0000-0003-4547-8094)
- Jonathan Allen
- A. Sundby
Institutions
- Ontario Institute for Cancer Research (CA)
- University Health Network (CA)
- Okayama University Hospital (JP)
Publication Details
- Journal
- JHLT Open
- Published
- 2025-11-06
- DOI
- https://doi.org/10.1016/j.jhlto.2025.100433
- Primary Topic
- Transplantation: Methods and Outcomes
- Type
- article
- Field-Weighted Citation Impact
- 0.00