Prediction and quality evaluation of quality markers of Gentiana scabra Bunge. in treatment of liver injury

The potential Q-markers of GSB were predicted through the integration of HPLC fingerprinting, pattern recognition, and small molecule-protein interaction analysis. Cell experiment results showed that swertiamarin, gentiopicroside, and sweroside significantly alleviated liver cell injury. Quantitative analysis of the Q-markers identified significant differences in their contents across GSB samples from different producing areas. This methodology provides a novel and comprehensive framework for defining Q-markers and evaluating GSB quality.