Characterization of the cell-free DNA released by tumor organoids derived from colorectal cancer patients
PDTOs release both nuclear and mitochondrial cfDNA into their culture medium displaying high similarity with patient-derived circulating DNA (cirDNA), including fragmentation patterns and oncogenic mutations. This study strengthens the relevance of the PDTOs as patient tumors models and highlights the potential of analyzing PDTO-derived cfDNA as a non-invasive approach to investigate tumor evolution and as a valuable tool to support functional precision oncology.
Authors
- Corinne Prévostel (ORCID: https://orcid.org/0000-0002-7553-087X)
- Ekaterina Pisareva (ORCID: https://orcid.org/0000-0002-0617-6889)
- Jérôme Cartry (ORCID: https://orcid.org/0000-0002-1693-1323)
- Alice Boilève (ORCID: https://orcid.org/0000-0003-3708-4909)
- Jacques R.R. Mathieu (ORCID: https://orcid.org/0009-0005-2239-6303)
- Fanny Jaulin (ORCID: https://orcid.org/0000-0002-5110-1800)
- Michel Ducreux (ORCID: https://orcid.org/0000-0001-8649-7449)
- Alexia Mirandola (ORCID: https://orcid.org/0009-0006-1761-1176)
- Alain R. Thierry (ORCID: https://orcid.org/0000-0002-4632-5404)
- Céline Gongora (ORCID: https://orcid.org/0000-0001-9034-4031)
- Sabrina Bedja
- Antoine Italiano
- Benjamin Ginter
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université de Bordeaux (FR)
- Inserm (FR)
- Université de Montpellier (FR)
- Université Paris-Saclay (FR)
- Institut Gustave Roussy (FR)
- Institut Bergonié (FR)
- Institute for Neurosciences of Montpellier (FR)
- Institut de Recherche en Cancérologie de Montpellier (FR)
Publication Details
- Journal
- European Journal of Cancer
- Published
- 2025-11-06
- DOI
- https://doi.org/10.1016/j.ejca.2025.116097
- Citations
- 2
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 1.87