Description of two novel species of the genus Chryseobacterium: Chryseobacterium chendengshani sp. nov. and Chryseobacterium wangxinyae sp. nov., isolated from soil of the Qinghai–Tibet Plateau

Four strains (LJ668^T/LJ756 and CY350^T/CY353) belonging to the genus <i>Chryseobacterium</i> were isolated from the Qinghai-Tibet Plateau soils. Cells were aerobic, Gram-stain-negative, rod-shaped, yellow-pigmented, non-motile and non-spore-forming. According to the 16S rRNA gene sequence alignment, the strain pair LJ668^T/LJ756 shared the highest similarities to <i>Chryseobacterium aahli</i> T68^T (98.09%) and <i>Chryseobacterium limigenitum</i> SUR2^T (98.09%), and the other one (CY350^T/CY353) to <i>Chryseobacterium aquaticum</i> KCTC 12483^T (97.98/98.13%). The phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that these four strains generated two separate clades in genus <i>Chryseobacterium</i>. The digital DNA-DNA hybridization and average nucleotide identity values of four novel isolates with other members of the genus <i>Chryseobacterium</i> were far below 70.0% and 95.0-96.0 % thresholds. The genomic DNA G+C content of LJ668^T and CY350^T is both 35.0 mol%. The major cellular fatty acids of the two pair strains are <i>iso</i>-C_{15 : 0} and summed feature 3 (C_{16 : 1} ω7c and/or C_{16 : 1} ω6c). MK-6 is the only respiratory quinone for strains LJ668^T and CY350^T. The polar lipids of strain LJ668^T are phosphatidylethanolamine (PE), L1-5 and AL1-5, and the strain CY350^T is PE, L1-4, AL1-2, diphosphatidylglycerol, phosphoglycolipid and GL1-3. According to the results of genotypic, phenotypic and biochemical analyses, these four strains represent two novel species of the genus <i>Chryseobacterium</i>, for which the names <i>Chryseobacterium chendengshani</i> sp. nov. and <i>Chryseobacterium wangxinyae</i> sp. nov. are proposed. The type strains are LJ668^T (=CGMCC 1.17165^T=JCM 33877^T) and CY350^T (=GDMCC 1.3790^T=KCTC 25690^T), respectively.