Hydrogen-driven, ATP-dependent biocatalytic reduction of carboxylic acids under non-explosive conditions
The reduction of carboxylic acids to alcohols is a key chemical transformation. We present a whole-cell biocatalyst employing carboxylic acid reductases and H<sub>2</sub> for cofactor regeneration. H<sub>2</sub> as a reductant ensures high atom and mass efficiency, enabling a more sustainable route to alcohols. Notably, we showcase that the reaction proceeds under O<sub>2</sub>-limited conditions, demonstrating potential for safer bioprocesses.
Authors
- Robert Kourist (ORCID: https://orcid.org/0000-0002-2853-3525)
- Margit Winkler (ORCID: https://orcid.org/0000-0002-0754-9704)
- Marianna Karava (ORCID: https://orcid.org/0000-0001-9147-4153)
- Qian Liang (ORCID: https://orcid.org/0009-0009-8050-8771)
- Elske van der Pol
Institutions
- Graz University of Technology (AT)
- Austrian Centre of Industrial Biotechnology (Austria) (AT)
Publication Details
- Journal
- Green Chemistry
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5gc03751d
- Citations
- 1
- Primary Topic
- Enzyme Catalysis and Immobilization
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- European Commission
- Austrian Science Fund
- HORIZON EUROPE Framework Programme
- Research Executive Agency