Quantification of eight clinically relevant serum adrenal steroids in infants by UHPLC-APCI-MS/MS
A novel, accurate, and sensitive UHPLC-APCI-MS/MS-based method for the simultaneous analysis of eight steroids in serum was developed, validated and successfully applied to infants younger than three months of age. The method enabled detection of abnormal values of precursors and steroid hormones in patients with adrenal disorders, with the potential for a large impact on paediatric patient care.
Authors
- María Gabriela Ropelato (ORCID: https://orcid.org/0000-0003-0040-1210)
- Marı́a Gabriela Ballerini (ORCID: https://orcid.org/0000-0003-0312-7421)
- Rodolfo Alberto Rey (ORCID: https://orcid.org/0000-0002-1100-3843)
- Manuela Martinefski (ORCID: https://orcid.org/0000-0002-4501-3783)
- Marı́a Eugenia Monge (ORCID: https://orcid.org/0000-0001-6517-5301)
- María E Rodríguez (ORCID: https://orcid.org/0000-0003-4746-8324)
- Verónica Ambao (ORCID: https://orcid.org/0009-0006-8806-2530)
Institutions
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Universidad de Buenos Aires (AR)
- Instituto de Química y Fisicoquímica Biológicas (AR)
- Hospital General de Niños Ricardo Gutierrez (AR)
Publication Details
- Journal
- Journal of Mass Spectrometry and Advances in the Clinical Lab
- Published
- 2025-11-05
- DOI
- https://doi.org/10.1016/j.jmsacl.2025.11.001
- Primary Topic
- Hormonal and reproductive studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00