Comprehensive genome analysis of Burkholderia gladioli BGLCN isolated from rice seedlings in Thailand reveals potential for secondary metabolite production

<i>Burkholderia gladioli</i> is a Gram-negative bacterium with multi-host adaptability as both a plant pathogen and an opportunistic human pathogen. It is also known as a producer of diverse bioactive metabolites. This dataset presents the first complete genome of <i>B. gladioli</i> isolated from Thailand, designated strain BGLCN, recovered from rice seedlings in Chai Nat Province. In preliminary assays, this strain exhibited <i>in vitro</i> antibacterial activity against human pathogens, including <i>Acinetobacter baumannii, Escherichia coli</i>, and <i>Staphylococcus aureus</i>, making it a promising candidate for the study of antibacterial compounds. The genome was sequenced using both Illumina NovaSeq and Oxford Nanopore PromethION platforms. A hybrid assembly generated two complete chromosomes and four plasmids with total genome size of 7.93 Mb with a GC content of 67.83 % and 100 % completeness. The genome contains 7266 coding sequences, 15 rRNA genes, and 84 tRNA genes. Phylogenomic analysis showed that BGLCN is closely related to rice-derived isolates from Vietnam. Biosynthetic gene cluster analysis using antiSMASH and DeepBGC revealed several putative secondary metabolite pathways, including polyketides, nonribosomal peptides, and saccharides. This dataset provides a valuable reference for comparative genomics, phylogenetic studies, and exploration of bioactive compound biosynthesis in <i>B. gladioli</i>. The genome is available under BioProject PRJNA1337644.