The optimal reference genes for RT-qPCR in various tissues of Cyperus rotundus L.
Background: Purple nutsedge (Cyperus rotundus L.), a perennial C4 plant from the Cyperaceae family, is one of the most invasive weed species worldwide and significantly affects crop yields. Objective: To identify and validate stable reference genes (RGs) for reliable gene expression normalization in RT-qPCR analyses across various tissues of purple nutsedge. Methods: The expression patterns of 11 candidate RGs were evaluated across three tissue types, including tubers at three developmental stages forming tubers (FT), swelling tubers (ST), and mature [...]
Authors
- Meisha Qiu (ORCID: https://orcid.org/0000-0002-5772-147X)
- Huihua Tan (ORCID: https://orcid.org/0000-0002-0085-2831)
- Yanhui Wang (ORCID: https://orcid.org/0000-0001-8309-6527)
- Jingbo Li (ORCID: https://orcid.org/0000-0002-4637-0731)
- H.D. Zhang (ORCID: https://orcid.org/0009-0005-8025-2009)
- Yonglin Ma (ORCID: https://orcid.org/0009-0006-9548-0116)
- W. Li (ORCID: https://orcid.org/0009-0003-0216-2636)
- Xiao Li (ORCID: https://orcid.org/0009-0009-1552-2622)
- Wei Zheng (ORCID: https://orcid.org/0009-0003-8872-8636)
Publication Details
- Journal
- Advances in Weed Science
- Published
- 2025-01-01
- DOI
- https://doi.org/10.51694/advweedsci/2025;43:00028
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Guangxi Academy of Agricultural Sciences