Training Effects on Changes in Differential Scanning Calorimetry (DSC) Profiles, Fluorescence Spectroscopy, and Circular Dichroism (CD) of Human Blood Serum

The study aimed to investigate the acute effects of a single leg press resistance training sessions that differed in training intensity on blood serum proteins using differential scanning calorimetry (DSC), circular dichroism (CD), and fluorescence spectra. Seven men with strength training experience participated in a randomized crossover trial, performing two experimental sessions. Each session included four sets of leg press-to-fall exercises, using loads equivalent to either 30% or 70% of their one-repetition maximum (1RM), with a 3 min rest interval between sets. Aqueous solutions of serum from blood samples taken at baseline (BA) and immediately postexercise (POST) were analyzed. The measurement techniques used allowed to observe postexercise changes in blood serum. Changes were observed in DSC profiles of blood serum, 3D fluorescence maps, and CD spectra. Statistically significant differences between stages "before" and "after" effort have been found for parameters of the endothermic transition associated with the denaturation of serum proteins. The results demonstrate the possibility of monitoring the effect of exercise on serum changes using DSC, CD, and fluorescence.