Digital droplet PCR quantification and field-scale spatial distribution of <i>Plasmopara viticola</i> oospores in vineyard soil

Grapevine downy mildew caused by the oomycete <i>Plasmopara viticola</i> affects leaves and bunches and leads to important economic losses for viticulturists. Recently, evidence has accumulated that soilborne primary inoculum (i.e., oospores in the soil) importantly contributes to disease progress. The significance of our work is in presenting a direct and sensitive method for assessing soil oospore concentration, as well as quantitative and spatially explicit data on downy mildew primary inoculum. This opens the way to new research, the evaluation of new disease control strategies based on primary inoculum management and the improvement of epidemic risk models, which will potentially contribute to lower fungicide use in viticulture <i>in fine</i>.

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Publication Details

Journal
Applied and Environmental Microbiology
Published
2025-11-04
DOI
https://doi.org/10.1128/aem.01667-25
Primary Topic
Horticultural and Viticultural Research
Type
article
Field-Weighted Citation Impact
0.00

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article

Digital droplet PCR quantification and field-scale spatial distribution of Plasmopara viticola oospores in vineyard soil

Isabelle D. Mazet, Mélanie Lelièvre, Eva Courchinoux, François Delmotte et al.
Applied and Environmental Microbiology
Horticultural and Viticultural Research
article

Digital droplet PCR quantification and field-scale spatial distribution of Plasmopara viticola oospores in vineyard soil

Isabelle D. Mazet, Mélanie Lelièvre, Eva Courchinoux, François Delmotte, Jean‐Pierre Rossi, Laurent Delière, Jean‐Pascal Goutouly, Alexandre Chataigner, M. Raynal, Marie Rodriguez, Charlotte Poeydebat
article en

Abstract

Grapevine downy mildew caused by the oomycete <i>Plasmopara viticola</i> affects leaves and bunches and leads to important economic losses for viticulturists. Recently, evidence has accumulated that soilborne primary inoculum (i.e., oospores in the soil) importantly contributes to disease progress. The significance of our work is in presenting a direct and sensitive method for assessing soil oospore concentration, as well as quantitative and spatially explicit data on downy mildew primary inoculum. This opens the way to new research, the evaluation of new disease control strategies based on primary inoculum management and the improvement of epidemic risk models, which will potentially contribute to lower fungicide use in viticulture <i>in fine</i>.

Applied and Environmental MicrobiologyVol. 91(12)
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR), Université de Bordeaux (FR), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR), École Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (FR), Santé et Agroécologie du Vignoble (FR), Unité mixte de recherche Œnologie (FR), Institut Agro Dijon (FR), Institut Agro Montpelier (FR), Centre de Biologie pour la Gestion des Populations (FR), Institut de Recherche pour le Développement (FR)
Agence Nationale de la Recherche, Analyses et Expérimentations pour les Ecosystèmes, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, CHIST-ERA
Openalex Percentile: Top 76%
Horticultural and Viticultural Research
DOIOpen Access PDFOpenAlex
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