Selenium-enriched Saccharomyces cerevisiae ameliorated copper-induced intestinal damage by modulating inflammation, oxidative stress, and immune response genes
Copper (Cu) toxicity induces intestinal mucosal damage in chickens. However, selenized-yeast (SY) is reported to enhance the general health and metabolic state of broiler chickens. Thus, this study was proposed to discover whether SY could protect chickens against Cu-induced intestinal damage. Healthy chicks were divided into 4 groups: Control group received basal diet; Cu group, received CuSO4 at 300 mg/kg, SY group received 0.4 mg/kg, and SY plus CuSO4 group received both treatments in diet. The current data indicated a significant reduction (P < 0.05) in residual Cu levels and MDA levels with a substantial increase in (P < 0.05) the activities of GPx, CAT, and SOD in the SY-supplemented birds compared to the Cu-challenged ones. Moreover, the reduced (P < 0.05) NO levels and inflammatory markers affirming the anti-inflammatory role of SY. Besides, SY co-administration significantly (P < 0.05) downregulated the AvBD-2, and AvBD-9 and upregulated (P < 0.05) the Gastrotropin, Calbindin, Cath-B, and Muc2 mRNA expressions compared to Cu-intoxicated group. The histopathological findings validated the protective effect of SY on Cu-induced intestinal damage Our research suggested that SY may be used as a feed supplement to protect the intestine injury during Cu exposure. This might be attributed to the antioxidant and anti-inflammatory potential of SY.
Authors
- Samah Fathy Ibrahim (ORCID: https://orcid.org/0000-0002-2824-3296)
- Ahmed Abdeen (ORCID: https://orcid.org/0000-0003-4907-955X)
- Heba Allah M. Elbaghdady (ORCID: https://orcid.org/0000-0002-2530-3847)
- Eman S. El‐Ashry (ORCID: https://orcid.org/0000-0001-7129-7815)
- Mohamed Elsayed Mohamed Mohamed (ORCID: https://orcid.org/0000-0002-9037-0253)
- Mohamed E. Salama (ORCID: https://orcid.org/0000-0001-6696-0061)
- Rana Morsi (ORCID: https://orcid.org/0000-0002-1660-9174)
- Ahmed Ateya (ORCID: https://orcid.org/0000-0002-2851-1350)
- Rehab Mady
- Donia Elsaid Fathi Zaghamir (ORCID: https://orcid.org/0009-0004-5689-7758)
- Ola A. Habotta (ORCID: https://orcid.org/0000-0003-0067-2838)
- N. Hashem (ORCID: https://orcid.org/0000-0002-3443-040X)
- S. Abed (ORCID: https://orcid.org/0000-0002-4937-1182)
- Ehab S. Taher
- Dania Abdelhady
- Eman Gadu (ORCID: https://orcid.org/0000-0001-8894-6068)
- Ahmad F. Rawan
Institutions
- Princess Nourah bint Abdulrahman University (SA)
- Alfaisal University (SA)
- Prince Sattam Bin Abdulaziz University (SA)
- Zarqa University (JO)
- Mansoura University (EG)
- Damanhour University (EG)
- Benha University (EG)
- Nangarhar University (AF)
- Dubai Medical College (AE)
- Almaarefa University
- Fayoum University (EG)
- Imam Abdulrahman Bin Faisal University (SA)
- Port Said University (EG)
Publication Details
- Journal
- Environmental Sciences Europe
- Published
- 2025-11-04
- DOI
- https://doi.org/10.1186/s12302-025-01098-6
- Primary Topic
- Selenium in Biological Systems
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Prince Sattam bin Abdulaziz University
- AlMaarefa University
- Princess Nourah Bint Abdulrahman University
- Zarqa University