Synergistic PEDOT:SBCD Electrochemical Platform for Simultaneous Detection of Multiple Metabolic Biomarkers in Human Urine
In this work, an electrochemical platform based on poly(3,4-ethylenedioxythiophene) doped with sulfonated β-cyclodextrin (PEDOT:SBCD) was developed for the simultaneous detection of ascorbic acid (AA), dopamine (DA), uric acid (UA), l-tyrosine (l-Tyr), and nitrite (NO<sub>2</sub><sup>-</sup>). The PEDOT:SBCD composite was synthesized via oxidative polymerization and characterized in detail. Afterward, under optimal conditions, the linear ranges were 5-4000 μM for AA, 0.1-250 μM for DA, 0.1-250 μM for UA, 5-1500 μM for l-Tyr, and 5-1000 μM for NO<sub>2</sub><sup>-</sup>, and the detection limits were 1.581 μM (AA), 0.061 μM (DA), 0.122 μM (UA), 0.629 μM (l-Tyr), and 0.510 μM (NO<sub>2</sub><sup>-</sup>), respectively. Notably, the platform achieved direct detection in real urine samples with minimal pretreatment, demonstrating excellent anti-interference capability and long-term stability. Additionally, it consistently detected target analytes in actual urine samples from volunteers of varying genders and age. This work provides a potential robust electrochemical platform for personalized and preventive healthcare.
Authors
- Xiaoliang Yu (ORCID: https://orcid.org/0000-0002-3596-2619)
- Qian Zhao (ORCID: https://orcid.org/0000-0003-0408-0864)
- Suqin Wang (ORCID: https://orcid.org/0000-0003-1725-6902)
- Shuzhu Zhang (ORCID: https://orcid.org/0000-0002-5961-0175)
- Zhi Fan (ORCID: https://orcid.org/0000-0002-5176-9712)
- Jiayu Zhang (ORCID: https://orcid.org/0009-0001-8003-9225)
- Yu Zhang (ORCID: https://orcid.org/0000-0001-6183-0612)
- Jin Zhao (ORCID: https://orcid.org/0000-0001-6291-6629)
- Jia Guo (ORCID: https://orcid.org/0000-0003-4589-2981)
- Wei‐Lei Zhou (ORCID: https://orcid.org/0009-0002-7867-5721)
- Zuhang Ding
Institutions
- Inner Mongolia University for Nationalities (CN)
- Jiangsu University (CN)
- Tianjin University of Science and Technology (CN)
- Tianjin University of Technology (CN)
- Mongolian University of Science and Technology (MN)
- Tianjin University (CN)
- Xuzhou Medical College (CN)
- Tianjin Medical University Cancer Institute and Hospital (CN)
- Xuzhou Cancer Hospital (CN)
- Xuzhou No.1 People's Hospital (CN)
- National University of Mongolia (MN)
Publication Details
- Journal
- ACS Macro Letters
- Published
- 2025-11-04
- DOI
- https://doi.org/10.1021/acsmacrolett.5c00582
- Citations
- 1
- Primary Topic
- Electrochemical sensors and biosensors
- Type
- article
- Field-Weighted Citation Impact
- 0.73