Healthy live birth after microsurgical enucleation of tripronuclear human zygote derived from ICSI: a case report
In the process of in vitro fertilization (IVF), zygote is considered as normally fertilized when there are two pronuclei (PN), one from sperm and the other from oocyte, and with two polar bodies (PBs). However, it is not uncommon to encounter tripronuclear (3PN) zygotes when conducting PN check for IVF or intracytoplasmic sperm injection (ICSI). Embryos derived from 3PN zygotes often lead to implantation failure, miscarriages and molar pregnancies, and these embryos are routinely discarded or not allowed to be transplanted. Here we report a case where most of the patient's oocytes (14/20) were immature (MI, GV), or with large or giant PB. After fertilization with ICSI, almost all resulting zygotes (7/8) were 3PN. By removing one female PN in 6 3PN zygotes, two microsurgically enucleated zygotes developed into high-quality blastocysts. One euploid blastocyst was selected for transfer after non-invasive chromosome screening (NICS), vitrification and thawing. The transfer resulted in the live birth of a healthy boy who is now 9-month-old and has no any health or developmental impairments.
Authors
- Fuxi Zhu (ORCID: https://orcid.org/0000-0001-8543-9028)
- Hai Xiang Lin (ORCID: https://orcid.org/0000-0002-1653-4854)
- Shumin Hu (ORCID: https://orcid.org/0000-0001-7889-9309)
- Xi Liao (ORCID: https://orcid.org/0009-0005-2752-7421)
- Huan Shen
- Yu Wang (ORCID: https://orcid.org/0000-0001-5107-8770)
- Shi Ren (ORCID: https://orcid.org/0000-0002-8212-7624)
- Shan Du
- Chunlai Zhang (ORCID: https://orcid.org/0000-0002-3502-6150)
- Xiaoming Xu (ORCID: https://orcid.org/0000-0001-7266-7611)
- Yun Luo (ORCID: https://orcid.org/0000-0003-0761-6521)
- Chun So (ORCID: https://orcid.org/0000-0003-3901-7654)
- Haowen Xu (ORCID: https://orcid.org/0000-0002-0455-5674)
- Xianbo Zuo (ORCID: https://orcid.org/0000-0002-0212-2664)
Institutions
- Anhui Medical University (CN)
- Beijing United Family Hospital (CN)
- National Institute of Biological Sciences, Beijing (CN)
- Shenyang 242 Hospital (CN)
- Second Affiliated Hospital of Anhui Medical University (CN)
- Tsinghua University (CN)
Publication Details
- Journal
- Journal of Ovarian Research
- Published
- 2025-11-04
- DOI
- https://doi.org/10.1186/s13048-025-01816-x
- Primary Topic
- Reproductive Biology and Fertility
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Directorate for Biological Sciences
- National Key Research and Development Program of China