Deciphering oligomeric proanthocyanidins’ dual osteoprotective mechanisms at single-cell resolution: NR4A1-mediated PTGS2 suppression and β-catenin-Runx2 activation
Our research integrated multilevel approaches, including single-cell transcriptomics, network pharmacology, cellular experiments, and animal models, to systematically reveal the dual mechanism of OPC in treating OP. This discovery not only established C2 <i>NR4A1</i>+ MSCs as key mediators in the pathological process of OP but also clarified the molecular mechanism of multitarget synergy of natural active compounds in restoring bone homeostasis, providing a theoretical basis and practical guidance for the development of new OP therapies.
Authors
- Yi Zheng (ORCID: https://orcid.org/0000-0002-2161-5480)
- Chunhan Sun
- Shaowei Zheng (ORCID: https://orcid.org/0009-0001-9064-8577)
- J. P. Zheng (ORCID: https://orcid.org/0000-0003-4891-0989)
- Shicheng Qiu (ORCID: https://orcid.org/0009-0009-0431-0982)
- Yirong Zeng (ORCID: https://orcid.org/0000-0003-3374-3161)
- Mingwei Chen (ORCID: https://orcid.org/0000-0002-8274-3099)
- Li Huang (ORCID: https://orcid.org/0000-0002-4495-598X)
- Yuwei Sun (ORCID: https://orcid.org/0009-0004-1580-0462)
Institutions
- Guangzhou University of Chinese Medicine (CN)
- Shandong University of Traditional Chinese Medicine (CN)
- Anhui Medical University (CN)
- First Affiliated Hospital of Guangzhou University of Chinese Medicine (CN)
- Jiangmen Wuyi Traditional Chinese Medicine Hospital (CN)
- First Affiliated Hospital of Anhui Medical University (CN)
- Huizhou Central People's Hospital (CN)
- Huizhou University (CN)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-11-04
- DOI
- https://doi.org/10.3389/fimmu.2025.1679987
- Primary Topic
- Nuclear Receptors and Signaling
- Type
- article
- Field-Weighted Citation Impact
- 0.00