Lgp-Cgf Improves Replantation Prognosis Of Avulsed Immature Permanent Teeth

To investigate the therapeutic effects of liquid/gel mixed phase concentrated growth factors (lgpCGF) on the healing of dental pulp and periodontal tissues in delayed replantation of avulsed immature permanent teeth in a Beagle dog model. The avulsion of immature permanent teeth in four 5.5 to 6.5 months old healthy purebred Beagle dogs were established by extraction, and the extracted teeth were dried for 60 minutes. The experiment was divided into the lgpCGF group and the conventional replantation group as control (n = 8). In the lgpCGF group, lgpCGF was prepared and injected into the alveolar socket before tooth replantation. Postoperative evaluations were conducted over four weeks, including micro-CT evaluation and histological analysis of dental pulp and periodontal tissues. Radiographic assessments revealed that a 10.46% increase in root length, a 35.30% reduction in apical diameter, and a 10.84% increase in root area in the lgpCGF group. Histological observations showed that lgpCGF application reduced the surface root resorption and inflammatory root resorption while promoting the alignment of periodontal ligament fibers. Additionally, pulp revascularization occurred more frequently in the lgpCGF group than those in the conventional replantation group, indicating superior pulpal healing. The application of lgpCGF in delayed replantation significantly improved pulp revascularization and periodontal ligament healing of avulsed immature permanent teeth. These findings suggest that lgpCGF is a promising therapeutic treatment for improving outcomes in delayed tooth replantation following dental trauma.