Gsdmd In Central Nucleus Of Amygdala Involved In Orthodontic Pain

Pain is the most common adverse reaction during orthodontic treatment. Patients only endure pain reluctantly, thus orthodontic doctors need pay more attention to recognize and solve this situation. Gasdermin D (GSDMD) is important in regulating pain, and the central amygdala (CeA) is also an important nucleus for pain regulation. Therefore, this study explored whether GSDMD in CeA could regulate orthodontic pain and the underlying mechanism. Adult C57BL/6J male mice were divided into control, sham and experiment group, this study approved by Institutional Animal Care Committee (No.2020-386). Behaviors were tested after experimental tooth movement (ETM) model established. And pseudorabies virus (PRV) was injected to periodontium to observe the virus expression in CeA. Then CeA tissues were collected for transmission electron microscope, immunofluorescence staining and western blotting analysis to detect the expression of GSDMD during tooth movement. Drug delivery via cannula, brain stereotaxic virus injection and electrophysiological techniques were comprehensively used to explore the undeilying mechanism. T-test, one-way ANOVA, and two-way ANOVA were used to analyze data. ETM resulted in obvious pain and anxiety-like behavior. Retrograde virus tracer indicated CeA can receive information from periodontium. Both staining and WB results showed that ETM resulted in a significant GSDMD and NF-κB/NLRP3 pathway protein expression increase in CeA. Furthermore, differential GSDMD/NLRP3 expression positively regulated pain and anxiety-like behavior through regulating the activity of neurons in CeA. GSDMD within CeA can positively regulate pain and anxiety behaviors during tooth movement.