Kdm4d Enhances Osteo/Dentinogenic Differentiation Of Dpscs Via Rps5

Dental pulp stem cells (DPSCs) hold great promise as candidates for pulp-dentin complex regeneration. Although significant progress has been made in understanding the transcriptional regulation of DPSCs' fate, the mechanisms governing their differentiation remain relatively poorly understood. To assess the osteo/dentinogenic differentiation capacity of Dental pulp stem cells (DPSCs), techniques such as alkaline phosphatase (ALP) activity assays, alizarin red staining, quantitative calcium analysis, and the detection of osteogenic markers were employed. Real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis were utilized to measure gene expression. The Seahorse Cell Mito Stress Test was carried out to detect the oxygen consumption rate (OCR), while the JC-10 assay was used to determine the mitochondrial membrane potential (MMP). And co-immunoprecipitation (Co-IP) assays were performed to explore the underlying molecular mechanisms. Finally, animal experiments were conducted to verify the effect of dental formation in vivo. It was found that KDM4D enhanced the osteo/dentinogenic differentiation, migration, and chemotaxis of DPSCs. The co-immunoprecipitation (Co-IP) results indicated that KDM4D could bind to RPS5. Intriguingly, RPS5 also contributed to promoting the osteo/dentinogenic differentiation, migration, and chemotaxis of DPSCs. KDM4D in combination with RPS5, promoted the osteo/dentinogenic differentiation and migration potential of DPSCs. This finding offers a valuable therapeutic clue for enhancing DPSC- based dental tissue regeneration.