Fzd7-Lrp5/6 In Hydrostatic Pressure Upregulated Chondrogenesis Of Bmscs

To detect the expression of Frizzled (FZD) 7-low-density lipoprotein receptor-related protein (LRP) 5/6 complex and chondrogenic marker genes in BMSCs under different mechanical environments. The self-developed multifunctional pressure loading system was used to treat the BMSCs under hydrostatic pressure of 0∼120 kPa for different times. The mRNA expression s of FZD7, LRP5/6, SOX9, Aggrecan, and COL-II in the cells were detected by real-time PCR. The protein levels of FZD7, LRP5/6, SOX9, Aggrecan, and COL-II were detected by Western blotting. Hydrostatic pressure of 0∼120 kPa for 1 hour, the mRNA expressions of FZD7, LRP5/6, SOX9, Aggrecan, and COL-II in BMSCs were higher than the blank control group and other experimental groups. The protein levels of FZD7, LRP5/6, SOX9, Aggrecan and COL-II were also higher than the blank control group and other experimental groups. When subjected to 0∼120 kPa of pressure for 1 hour per day, continuously for 1 day, the mRNA expressions of FZD7, LRP5/6, SOX9, Aggrecan, and COL-II in BMSCs were all higher than the blank control group and other experimental groups. The protein levels of FZD7, LRP5/6, SOX9, Aggrecan and Col-II were also all higher than the blank control group and other experimental groups. Hydrostatic pressure promotes the differentiation of BMSCs into chondrocytes. FZD7-LRP5/6 complex play a key role in upregulating the chondrogenic responses of BMSCs under hydrostatic pressure.