Periapical Tissue Reaction To Extruded Bioceramic Sealers On Rabbit Model

To evaluate the response of periapical tissues to the overfilling root canals with bioceramic sealers and epoxy resin-based sealer on a rabbit model. Eighteen male rabbits were used in the study, originating 3 experimental groups: G1- AH Plus (Dentsply DeTrey, Germany); G2 - TotalFill BC Sealer (FKG Dentaire SA, Switzerland); G3 - Bioceramin (Vladmiva, Russia). The materials were extruded into periapical tissues.The animals were killed by anesthetic overdose 7 and 30 days after endodontic treatment. The bone fragments with the treated incisor were obtained and serial histological sections were prepared for histomorphological study. Data were statistically analyzed by Wilcoxon nonparametric test at 5% significance level. The biocompatibility of sealers had a statistically significant difference between groups in 7-day period (p<0.01). Epoxy resin-based sealer showed a severe periapical tissue reaction for all observation periods. At the 7-day observation, group 2 and 3 showed an initial mild to moderate inflammatory periapical reaction. The only group to show a statistically significant reduction in inflammation during the 30-day period was the TotalFill BC Sealer (p>0.05). After 30-day observation period, TotalFill BC Sealer was less toxic than AH Plus and Bioseal. Bioceramic sealer TotalFill BC Sealer proved to have ideal characteristics for biocompatibility with periapical tissues. AH Plus sealer remained toxic for the duration of the study.