The Biomimetic Mineralized Amyloid Hydrogel For Enamel Ordered Biomineralization

Targeting at the key factors in the natural formation of enamel, the study plans to use polyethylene glycol (PEG)-modified bovine serum albumin (BSA-PEG) to regulate the β-sheet conformation, enabling the synchronous self-assembly of BSA-PEG and amorphous calcium phosphate (ACP) to construct the biomimetic mineralization hydrogel. The rapid amyloid transformation of BSA-PEG was induced by tris(2-carboxyethyl)phosphine (TCEP), the reaction kinetics process was monitored by fluorescence spectrophotometer and the amyloid transformation of BSA was detected by fourier transform infrared spectroscopy (FTIR). The surface remineralization effect of enamel was observed by SEM, X-ray diffraction (XRD) was employed to characterize whether the remineralized crystal was HA, and the changes of mineralization degree before and after mineralization was detected by a microhardness tester. The use of TCEP induces rapid amyloidosis in the BSA-PEG complex. The results of FTIR show that the protein secondary structure was transformed from α-helix to β-sheet, fluorescence monitoring shows the formation of amyloid fibril structure and the biomimetic mineralization hydrogel is successfully prepared. The hydrogel can induce dense mineralized layer on the surface of enamel. XRD results show that the newly formed mineral crystals were HA, which can restore enamel hardness to a greater extent than the fluoride positive control. The biomimetic mineralized amyloid hydrogel has been prepared successfully on enamel and has good induced remineralization and antibacterial effects. The implementation of this study is expected to provide new strategies for achieving ordered enamel mineralization and caries prevention and treatment.