Mir-129-1-3P Inhibits Osteogenic Differentiation Of hBMSCs

To investigate the effect of miR-129-1-3p on the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and its potential mechanism. Negative control group, the miR-129-1-3p mimic group, the miR-129-1-3p inhibitor group and the corresponding negative control were constructed and transfected into hBMSCs. The formation of calcium-mineralized nodules was observed by alkaline phosphatase (ALP) staining and alizarin red S (ARS) staining. The expression levels of miR-129-1-3p and osteogenic differentiation markers were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot (WB) detected the protein expressions of bone morphogenetic protein 2 (BMP2), SMAD1, p-SMAD1. After transfection, the expression level of miR-129-1-3p in mimic group was significantly increased (p<0.05), the number of mineralized nodules was significantly decreased, and the expression levels of BMP2, Runt-related transcription factor 2 (RUNX2), osteocalcin (OCN) mRNA were significantly down-regulated (p<0.05), BMP2 and p-SMAD1 protein were also significantly down-regulated (p<0.05) compared with mimic-NC group. The expression levels of BMP2, RUNX2, OCN mRNA were significantly up-regulated in inhibitor group (P<0.05) compared with inhibitor-NC group, consistently, BMP2 and p-SMAD1 protein were significantly up-regulated in inhibitor group (P<0.05). miR-129-1-3p can inhibit the osteogenic differentiation of human bone marrow mesenchymal stem cells by suppressing BMP2/SMAD1 signaling pathway.