Pth's Impact And Mechanism On Alveolar H-Type Angiogenesis.

Accelerating the healing of alveolar bone defects from oral infections is a key focus in osteogenesis research. Bone-specific H vessels and their vascular pericytes are crucial for regulating bone formation and maintaining balance. Parathyroid hormone (PTH) has been shown to enhance H-type blood vessel and pericyte formation in long bones, promoting osteogenesis. However, the mechanism and its effects on alveolar bone require further investigation. 20 8-week-old C57BL6/J male mice were randomly divided into PTH injection group and control group. PTH (20 μ g / kg/day) or normal saline was injected twice a day. 14 days later, bilateral leg and mandible tissues were fixed and decalcified to make paraffin sections or preserved in liquid nitrogen to extract RNA and total protein. Proteomic antibody microarray, HE and immunohistochemical staining and real-time PCR were performed. MS1 endothelial cells and MC3T3-E1 cells were cultured together in vitro. Scratch test, migration test and tube formation test were carried out under the condition of PTH stimulation. Total RNA and mi-RNA were extracted and real-time PCR was used to detect vascular related genes. H-type blood vessels exist in alveolar bone, and PTH stimulation can increase the number of H-type blood vessels and pericyte, potentially enhancing osteogenesis via the HIF1 α / MALAT1/VEGFA signaling pathway. Moreover, PTH injection modifies the bone marrow's immune environment, promoting osteogenesis. PTH stimulation may enhance osteogenesis by increasing H-type blood vessels and pericyte in bone marrow microenvironment.