Preliminary Study On The Prerequisites For Mineralization Via Pccp Process

This study aimd to investigate the binding ability of metal ions with polyaspartic acid (PAsp), PAsp-metal ion complexes solubility, metal hydroxide solubility, suspension stability of cation liquid and binding ability of anions in phosphate liquid with metal ions as well as to summarize the prerequisites of mineralization via polyelectrolyte-calcium complexes pre-precursor (PCCP) process. Binding ability of metal ions and PAsp was analyzed with isothermal titration calorimetry and circular dichroism spectra; PAsp-metal ion complexes solubility were analyzed by inductively coupled plasma-mass spectrometry; Metal hydroxide solubility was analyzed by potentiometric titration; Suspension stability was analyzed with multifunctional microplate reader; Binding ability of anions and metal cations was simulated by Visual minteq 4.0 software. Intrafibrillar mineralization was analyzed with transmission electron microscopy and energy dispersive spectroscopy-mapping. Based on results above, prerequisites of mineralization via PCCP process were summarized. Hardly mineralizable: Cation liquid: Metal ions and PAsp had no or weak binding ability; Anion liquid: Anions had weak binding ability with metal ions. Moderately mineralizable: Cation liquid: Metal ions and PAsp had certain binding ability; Anion liquid: Anions had strong binding ability with metal ions. Easily mineralizable: Cation liquid: Metal ions and PAsp had moderate binding ability; Metal hydroxides had certain solubility; PAsp-metal ion complexes had high solubility; Cation liquid had moderate stability; Anion liquid: Anions had strong binding ability with metal ions. Prerequisites of mineralization via PCCP process can assist in predicting what kinds of metal ions could be used for antibacterial biomimetic mineralization.